Acoustic levitation is a non-linear effect under the high sound intensity condition. The principle is that the vertical levitation force is generated by the interaction between acoustic standing wave and object to overcome the weight of small object, and the horizontal positioning force is generated to fix the object at the node of sound wave. The standing wave field is very sensitive to the distance between resonantor and difficult to manipulate particles. In the paper a non-resonant standing wave field is used for acoustic levitation to manipuated particle in air. A series of experiments are investigated and discussed.